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Dispatches based on the variant of the result of val-expr , where val-expr must have type tyid/abs , and tyid/abs must refer to a type defined via define-type . The result is the value of expr for the variant-id that is instantiated by val-expr or the expr in an else clause if no variant-id matches. Each field-id is bound to a corresponding (by position) value of a field within the variant instance for use in the same clause’s expr .

The number of field-id s must match the number of fields declared for variant-id in the definition of tyid/abs , and either every variant-id of tyid/abs must have a clause in the Free Shipping Cost Wholesale factory custom 7 color glowing running led light up shoes Choice Cheap Online Cheap Finishline Sast Cheap Price Cheap Sale 100% Original vOfO6SmS
form or an else clause must be present.

Either returns expr ’s result or catches an exception raised by expr and calls handle-expr .
The test form checks whether the value of the first expr matches the value of the second expr , and reports a test failure or success. If the results of the two expr s are inexact numbers, the test passes as long as the difference between the number is less than 0.01 .

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form checks whether the expr raises an exception whose error message includes the string produced by string-expr .

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type merely prevents your program from using the result.

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The sequencing data alone do not demonstrate overexpression of the FoxP2 isoforms. Quantification of the cells infected at the injection site using the identical procedures to ours here (see Heston White, 2015) indicates 20-25% of cells, the majority of which are FoxP2+ medium spiny neurons, are infected by the virus. Therefore, in our Area X samples, 70-75% of the medium spiny neurons are not overexpressing FoxP2. This presents a challenging situation: a clear and reproducible behavioral effect is observed with the virus manipulation, but the transcriptional basis for it is only visible in the qRT-PCR data presumably due to the amplification required to overcome a low signal-to-noise ratio.

We respectfully disagree regarding the necessity to present detailed differential expression analyses between Area X and VSP. In this study and in our prior work (Hilliard et al., 2012), differential expression between Area X and VSP are presented directly adjacent to figures plotting differential connectivity (see main text Figure 4D ). Our data indicate that a gene’s regionality and/or relevance to behavior is captured more effectively by considering its transcriptional context (i.e. connectivity) through network analysis instead of through changes in absolute expression level. Moreover, our expression data will be made available through GEO upon the manuscript’s publication so that interested parties can conduct differential expression analyses.

As indicated above, we find no evidence of FoxP2 overexpression in VSP either by: (1) in situ analysis, (2) examination of RNA-seq data from VSP punches, or (3) now in response to the Reviewer, qRT-PCR ( Wholesale latest soft sole casual tennis shoes running shoe for men Geniue Stockist Cheap Price Cheap Sale New beE7TovB90
, above).

In response to the reviewer’s request, we present figures below depicting how our samples relate to each other. When samples from both Area X and VSP are considered together, the samples clearly separate by brain region (see Author response image 6 ).

Within the Area X samples, grouping is not clearly delineated by viral construct (see Author response image 7 ). This is likely the product of a fairly subtle manipulation (<25% cellular transduction), noted above. Further, as described in this manuscript and our prior work (Hilliard et al., 2012), the act of singing has a dramatic effect on the transcriptome, correlating with changes in expression of thousands of genes. The animals in this study sang across a wide continuum (0 to ~1900 motifs) in the two hours immediately preceding sacrifice. Thus, our study does have biological replicates within the virus groups, but there are no replicates for singing since the animals were allowed to sing ad libitum before sacrifice. This variability is ideal for gene network analysis but creates the situation where a virus by singing interaction may occur, driving the clustering pattern we observe here.

Author response image 6
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One VSP sample (White383V) clustered with the Area X samples but was not included in the Area X network.

Author response image 7
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Virus construct is indicated in green (GFP), black (FoxP2.FL), and red (FoxP2.10+). Below, time singing is indicated on a blue-red color scale where the samples from birds that sang the most are …

We agree that the most compelling demonstration is to find the ‘learning modules’ in normal juvenile birds. Thanks to the reviewer’s point which was shared with the other reviews, this has now been demonstrated using birds injected with the GFP-expressing virus. Going further and as also requested, we present new data from chromatin immunoprecipitation experiments indicating that FoxP2 binds the MAPK11 promoter, providing a mechanistic basis for a prediction made by our network data. The results of these experiments have been added to the main text in the subsection “A bioinformatics approach indicates MAPK11 as an entry point to neuromolecular networks for vocal learning” and in Figure 6D . The methods for these experiments have been added to the Materials and methods section of the manuscript. It is our hope that by publishing the manuscript we can share the dataset with the scientific community enabling additional follow up on the highlighted pathways.

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expression is the result of the last body . The parameter-expr s determine the parameters to set, and the value-expr s determine the corresponding values to install while evaluating the body-expr s. All of the parameter-expr s are evaluated first (and checked with parameter? ), then all value-expr s are evaluated, and then the parameters are bound in the continuation to preserved thread cells that contain the values of the value-expr s. The last body-expr is in tail position with respect to the entire RAZAMAZA High Heel Thick Heel Sandal Summer Vacation Shoes Free Shipping Fast Delivery The Cheapest Online Sneakernews Sale Online QKHYj6PmUh

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expression, parameters remain bound to other thread cells. Effectively, therefore, old parameters settings are restored as control exits the Finishline For Sale New Bollywood Mouni Roy Designer White Suit Fashionable Anarkali Salwaar Kameez Bridal Wear Heavy Material Georgette Dress R1368 Fast Shipping 0QVmr

If a continuation is captured during the evaluation of parameterize , invoking the continuation effectively re-introduces the parameterization , since a parameterization is associated to a continuation via a continuation mark (see Continuation Marks ) using a private key.

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is the same as a nested series of single-parameter parameterize forms.
Returns a parameter procedure that sets or retrieves the same value as parameter , but with:

guard applied when setting the parameter (before any guard associated with parameter ), and

wrap applied when obtaining the parameter’s value.

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, which can also be used to guard parameter procedures.

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